hypDas a Marker for [NiFe]-Hydrogenases in Microbial Communities of Surface Waters

hypD as a marker for [NiFe]-hydrogenases in microbial communities of surface waters.

Hydrogen is an important trace gas in the atmosphere. Soil microorganisms are known to be an important part of the biogeochemical H2 cycle, contributing 80 to 90% of the annual hydrogen uptake. Different aquatic ecosystems act as either sources or sinks of hydrogen, but the contribution of their microbial communities is unknown. [NiFe]-hydrogenases are the best candidates for hydrogen turnover ...

Metal insertion into NiFe-hydrogenases.

The synthesis and the insertion of the metallocentre of NiFe-hydrogenases is a complex process, in which seven maturation enzymes plus ATP, GTP and carbamoyl phosphate are involved. The review summarizes what is known about the properties and activities of these auxiliary proteins, and postulates a pathway along which maturation may take place.

Mechanism of hydrogen activation by [NiFe] hydrogenases.

The active site of [NiFe] hydrogenases contains a strictly conserved arginine that suspends a guanidine nitrogen atom <4.5 Å above the nickel and iron atoms. The guanidine headgroup interacts with the side chains of two conserved aspartic acid residues to complete an outer-shell canopy that has thus far proved intractable to investigation by site-directed mutagenesis. Using hydrogenase-1 from E...

Computational studies of [NiFe] and [FeFe] hydrogenases.

Crystal Structure Determination of Hyp Proteins for [NiFe] Hydrogenases

Introduction [NiFe] hydrogenases catalyze the reversible oxidation of molecular hydrogen and carry a NiFe(CO)(CN)2 metal center at the active site. The biosynthesis of this metal center requires specific maturation proteins, HypA, HypB, HypC, HypD, HypE, and HypF [1]. HypA and HypB are involved in the insertion of the Ni atom into the precursor large subunit, as [NiFe] hydrogenases are composed...